Cyclophilin D

Cyclophilin D (CyPD) is a member of the immunophilin family of proteins. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents-cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyPD is located in the inter-membrane space of the mitochondria where it has been shown to be an integral member of the permeability transition pore complex which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml, detects a ~15kD protein in rat liver and heart extract, and a ~37 in rat brain extract.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Rat brain lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8855-20A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat, Can

Form

Supplied as a liquid in PBS, 1 mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Halestrap AP et al. Mitochondria and cell death. Biochem Soc Trans 28:170-7 (2000). <PUBMED:10816121>Andreeva L et al. Cyclophilins and their possible role in the stress response. Int J Exp Pathol 80:305-15 (1999). <PUBMED:10632780>

Additional Info

Recognizes human Cyclophillin D. Species Crossreactivity: rat, canine and hamster.