Cyclophilin D (PPID, Peptidylprolyl Isomerase D, 40kD Peptidyl-Prolyl cis-trans isomeras, Cyclophilin-40, Cyclophilin-Related Protein, CYP40, CYP-40, CYPD, MGC33096, PPIase, Rotamase)

Cyclophilin D (CyPD) is a member of the immunophilin family of proteins. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents-cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyPD is located in the inter-membrane space of the mitochondria where it has been shown to be an integral member of the permeability transition pore complex which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT).\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other Applications not tested.\n\nRecommended Dilutions:\nELISA: 1:64,000\nWestern Blot: 0.1-0.3ug/ml\nOptimal dilutions to be determined by the researcher\n\nPositive Control:\nHuman Brain (Frontal Cortex) lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See C8855-20B-P. Antibody

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SPECIFICATIONS

Catalog Number

C8855-20B

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

Forte M, Gold BG, Marracci G, Chaudhary P, Basso E, Johnsen D, Yu X, Fowlkes J, Bernardi P, Bourdette D. \nCyclophilin D inactivation protects axons in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7558-63. Epub 2007 Apr 26.

Additional Info

Recognizes human PPID at ~40kD. Species sequence homology: mouse, rat, canine.