CYP11A (Cholesterol Side-chain Cleavage Enzyme, Cytochrome P450 Family 11 Subfamily A)

Steroid hormones, including mineralocorticoids, glucocorticoids, and sex steroids, control the balance of ions in the blood; metabolism of carbohydrates, lipids, and proteins; and sexual characteristics, respectively. In steroidogenic tissues, such as the adrenal cortex, testis, ovary, and placenta, all steroids are synthesized from the common precursor cholesterol. Two families of steroidogenic enzymes, cytochrome P450 enzymes (CYP) and hydroxysteroid dehydrogenases (HSD), catalyze the production of most steroids. All six distinct steroid hydroxylases are cytochrome P450 enzymes encoded by the steroidogenic CYP gene family (1). The cytochrome P450scc (cholesterol side-chain cleavage enzyme) encoded by CYP11A1 catalyzes the first step in steroidogenesis, conversion of cholesterol into pregnenolone, and thus controls the rate of synthesis of all steroid hormones (2).CYP11A1, located in the inner membrane of mitochondria, cooperates with two coenzymes, ferredoxin and ferredoxin reductase, to carry out three successive oxidation-reduction reactions of cholesterol through an electron transport chain using NADPH as an electron donor (3-5). Like all CYPs, CYP11A1 expression is increased by the cAMP-PKA pathway (6) and the transcription factor SF1/NR5A1 has been shown to play a central role in mediating the cAMP signal on the CYP11A1 promoter (7). Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46, XY sex reversal (AICSR), which is a rare disorder that can present as acute adrenal insufficiency in infancy or childhood (8,9).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

137427

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Nelson, D.R. et al. (1993) DNA Cell Biol 12, 1-51. 2. Richards, J.S. et al. (1987) Recent Prog Horm Res 43, 231-76. 3. Hanukoglu, I. and Jefcoate, C.R. (1980) J Biol Chem 255, 3057-61. 4. Hanukoglu, I. et al. (1981) J Biol Chem 256, 4329-35. 5. Hanukoglu, I. et al. (1981) J Biol Chem 256, 4321-8. 6. Hu, M.C. et al. (1991) Biochem J 274 ( Pt 3), 813-7. 7. Watanabe, N. et al. (1994) Eur J Biochem 222, 825-34. 8. Tajima, T. et al. (2001) J Clin Endocrinol Metab 86, 3820-5. 9. Katsumata, N. et al. (2002) J Clin Endocrinol Metab 87, 3808-13.

Additional Info

Recognizes endogenous levels of total human CYP11A1. Species Crossreactivity: mouse, rat.