Cyr61 (Cysteine-rich angiogenic inducer 61), also known as IGFBP-10 and CCN1, is a 42kD secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins avb3, avb5, amb5 and a6b1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Mature human Cyr61 shares 93% aa sequence identity with mouse and rat Cyr61. Cyr61 expression appears to be unregulated in breast cancer cells with high levels of heregulin.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:250-1:1000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
