Stefin A is the intracellular inhibitor of cysteine proteinase cathepsin B (CatB) and belongs to family 1 of the cystatin superfamily of inhibitor proteins. It has been proposed that extracellular CatB together with other classes of proteinases are all subject to a cascade-like manner of activation. CatB and its inhibitor Stefin A are involved in degradation of extracellular matrix proteins during tissue remodeling. Stefin A had been reported to be in high concentrations in epithelial cells, polymorphonuclear leukocytes and lymphoid tissue.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Appendix
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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