CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000-1:3,000
Western Blot: 1:1000-1:3,000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CSTB protein.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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