Cystatin C (AD8, Amyloid Angiopathy and Cerebral Hemorrhage, Cst3, Cystatin 3, Gamma Trace, HCCAA, Neuroendocrine Basic Polypeptide, Post gamma Globulin, ARMD11, MGC117328, OTTHUMP00000030440, OTTHUMP00000164181, bA218C14.4)

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36kD and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's di-sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum \nCystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stabillty:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8950-11B

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

igG

References

1. Page M.K. et al.: . Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 \n(2000) . \n2. Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following \nphotothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. Epub 2005 Nov 23. \n3. Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. \nRen Fail. 2001 May-Jul;23(3-4):431-8. \n4. Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y \nAcad Sci. 2002 Nov;977:315-21. \n5. Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C \nprevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol \nDis. 2005 Feb;18(1):152-65. \n6. Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica \nChimica Acta 297, 55-66 (2000) . \n7. Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. \n8. Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by \neggwhite cystatin. Biochem J. 223, 245-253 (1984) . \n9. Lindahl P., Abrahamson M., Bjork L.: . Interaction of recombinant human cystatin C with the \ncysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) . \n10. Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp \nPhysiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24.

Additional Info

Recognizes mouse Cystatin C.