Cystatin C BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Cystatin C in cell culture supernates, serum, plasma (heparin, EDTA) and urine.\n\nCystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.\n\nAssay Range:\n312pg/ml-20,000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP21460\n\nEntrez Gene ID:\n13010\n\nKit Components:\n96-well plate pre-coated with anti-Cystatin C, Mouse\nLyophilized recombinant Cystatin C, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143803

Size

96Tests

References

1. Grubb, A.; Lofberg, H. : Mouse gamma-trace, a basic microprotein: amino acid sequence and presence in the adenohypophysis. Proc. Nat. Acad. Sci. 79: 3024-3027, 1982.\n2. Pirttila, T. J.; Manninen, A.; Jutila, L.; Nissinen, J.; Kalviainen, R.; Vapalahti, M.; Immonen, A.; Paljarvi, L.; Karkola, K.; Alafuzoff, I.; Mervaala, E.; Pitkanen, A. : Cystatin C expression is associated with granule cell dispersion in epilepsy. Ann. Neurol. 58: 211-223, 2005.\n3. Abrahamson, M. : Mouse cysteine proteinase inhibitors: isolation, physiological importance, inhibitory mechanism, gene structure and relation to hereditary cerebral hemorrhage. Scand. J. Clin. Lab. Invest. 48 (suppl. 191): 21-31, 1988.\n4. Pirttila, T. J.; Manninen, A.; Jutila, L.; Nissinen, J.; Kalviainen, R.; Vapalahti, M.; Immonen, A.; Paljarvi, L.; Karkola, K.; Alafuzoff, I.; Mervaala, E.; Pitkanen, A. : Cystatin C expression is associated with granule cell dispersion in epilepsy. Ann. Neurol. 58: 211-223, 2005.\n