Cystatin F, Recombinant, Mouse (CMAP, CST7, Cystatin 7, Leukocystatin, Cystatin-like Metastasis-associated Protein)

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a new member of the Cystatin superfamily (1-3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards Cathepsin L and legumain (1, 2, 5). Compared to other secreted Cystatins including C, D, E/M, S, SA and SN, which contain two intrachain disulfide bonds, Cystatin F has two extra Cys residues that may be involved in interchain disulfide bonds. rmCystatin F and rhCystatin F exhibit disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human Cystatin F and glutathione S-transferase (2).\n\nRecombinant mouse Cystatin F encoded by the Cst7 gene (rmCystatin F; amino acid residues 19 to 144; Accession # O89098) was expressed with an N-terminal signal sequence and a C-terminal 6X His tag in a murine myeloma cell line, NS0.\n\nCalculated molecular mass of 15kD and migrates as a 20kD band in SDS-PAGE under reducing conditions.\n\nActivity: Measured by its ability to inhibit human cathepsin L.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8950-22D

Size

10ug

Form

Supplied as a liquid in 25mM Tris, 0.15M NaCl, pH 7.5.

Purity

(same/more than) 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: (same/less than) 1EU/1ug.

References

1. Ni, J. et al., 1998, J. Biol. Chem. 273:24797.\n2. Halfon, S. et al., 1998, J. Biol. Chem. 273:16400.\n3. Utsunomiya, T. et al., 2002, Clin. Cancer 8:2591.\n4. Werle, B. et al., 2003, Biol. Chem. 384:281.\n5. Gruninger-Leitch, F. et al., 2000, Nat. Biotechnol. 18:66.