Cyr61, also known as IGFBP-10 and CCN1, is a 50kD secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins aVB3, aVB5, aMB2, and a6B1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% aa sequence identity with human and rat Cyr61, respectively.
Applications:
Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse Cyr61 is ~5ng/well.
Western Blot: 2ug/ml
Immunohistochemistry: 25ug/ml detects Cyr61 in frozen mouse trigeminal ganglia tissue sections.
Optimal dilutions to be determined by the researcher.
Western Blot Buffers:
Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride
0.02% Tween-20
Blocking Solution: 5% nonfat dry milk in blotting buffer
Antibody Solution: 5% nonfat dry milk in blotting buffer
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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