Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:500. Frozen and paraffin sections
Western Blotting: 1:5000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.