Cysteine-Rich Secretory Protein 3 (CRISP3)

CRISP-3 belongs to the large CRISP family of secreted proteins with a characteristic C-terminal cysteine-rich (CR) domain. Members of the CRISP family contain three conserved domains: a plant pathogenesis related group 1 (PR-1) domain, a short hinge region, and a CR domain. CRISP-3 is expressed in salivary glands, prostate, pancreas, and in the secretory granules of neutrophilic granulocytes. In plasma, CRISP-3 circulates in a complex with alpha B-glycoprotein, a member of the Ig superfamily. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds. Mammalian members of the CRISP family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, post-testicular sperm maturation and capacitation to oocyte-sperm fusion. CRISP3 is epithelium-specific and found predominantly in salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. CRISP3 is up-regulated in malignant prostatic epithelium, therefore, it can be used as a potential prostate cancer biomarker.\n\nApplications: \nSuitable for use in ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nELISA (competitive): 1:200-1:400\nFlow Cytometry: 1.2ug per 10e6 cells\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9018-58B

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1

References

1. Kra?tzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD (1996). The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 Eur J Biochem 15;236(3):827-36 2. Udby L, Bjartell A, Malm J, Egesten A, Lundwall A, Cowland JB, Borregaard N, Kjeldsen L (2005). Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract. J Androl ;26(3):333-42 3. Kosari F, Asmann YW, Cheville JC and Vasmatzis G (2002). Cysteine-rich Secretory Protein-3: A Potential Biomarker for Prostate Cancer. Cancer Epidemiology Biomarkers & Prevention 11;1419- 1426

Additional Info

Recognizes human native CRISP3 expressed on transfected mammalian cells