Cysteine String Protein (CSP)

Cysteine string protein (CSP) is a 34kD rat brain protein localized to the cytoplasmic surface of the synaptic vesicle, possibly via palmitoylation (1,2). CSP homologs are localized to the nervous system of Drosophila and to cholinergic vesicles of Torpedo (3,4). The amino terminus of CSP contains the J domain, a consensus sequence found on a DnaJ like protein which is a member of the heat-shock protein family (5). Since heat shock proteins serve as chaperones that participate in protein folding and protein complex formation, CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking. Evidence for a role of CSP in presynaptic neurotransmitter release has come from genetic and physiological study in which the Drosophila CSP null mutant fails to elicit synaptic potential upon high frequency stimulation (6). CSP null mutants have a lethal phenotype and for the small percentage of mutant flies that survive, they become paralyzed and die at elevated temperature (7).\n \nApplications:\nWestern Blot (Colorimetric) (1,8): 0.1ug/ml 1:1,000 \nImmunoprecipitation: 0.5ug/ml \nImmunohistochemistry: 1ug/ml \nImmunoelectron Microscopy (9) \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nMouse Brain Tissue Extract \nRat Brain Tissue Extract

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SPECIFICATIONS

Catalog Number

C9020-01

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, Bov, Xen/Amph

Form

Supplied as a liquid in borate buffered saline, pH 8.2 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Baun, J.E.A. and Scheller, R. (1995) Neuropharmacology 34: 1361-1369. \n2. Gundersen, C.B., Mastrogiacomo, A., Faull, K., & Umbach, J.A. (1994) J. Biol. Chem. \n269: 19197-19199. \n3. Mastrogiacomo, A., Parsons, S.M., Zampighi, G.A., Jenden, D.J., Umbach, J.A. & \nGundersen, C.B. (1994) Science 263: 981-982. \n4. Zinsmaier, K.E., Hofbauer, A., Heimbeck, G., Pflugfelder, G.O., Buchner, S., & Buchner, \nE. (1990) J. Neurogenet. 7: 15-29. \n5. Georgopoulos, C. & Welch, W.J. (1993) Ann. Rev. Cell Biol. 9: 601-634. \n6. Umbach, J.A., Zinsmaier, K.E., Eberle, K.K., Buchner, E., Benzer, S., & Gundersen, C.B. \n(1994) Neuron 13: 899-907. \n7. Zinsmaier, K.E., Eberle, K.K., Buchner, E., Walter, N., & Benzer, S. (1994) Science 263: \n977-980. \n8. Thomas, D.D.H., Taft, W.B., Kaspar, K.M., Groblewski, G.E. (2001) J Biol Chem 276(31): \n28866-28872. \n9. Sakisaka, T., Meerol, T., Matteson, J., Plutner, H., Balch, W.E. (2002) EMBO J 21(22): \n6125-6135.

Additional Info

This immunoaffinity purified antibody detects a 34kD protein, corresponding to the apparent molecular mass of CSP on SDS-PAGE immunoblots, in samples from mouse, rat, bovine and Xenopus. A 70kD band is also observed, which may represent a CSP \ndimer. No reactivity was seen with mouse liver extracts. The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.