Cytochrome B245 Heavy Chain (Cytochrome b-245 Heavy Chain, CGD, CGD91-phox, Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, CYBB, gp91-1, GP91PHOX, gp91-phox, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, NOX2, Neutrophil Cyt

The heavy chain of cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. \n\nApplications:\nSuitable for use in Flow cytometry. Other applications have not been tested. \n\nRecommended Dilutions: \nFlow cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Membrane permeabilisation is required for this application. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9094-61

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide, 1% BSA. Labeled with FITC.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Burrit, J. B. et al. (2003) Functional epitope on human neutrophil flavocytochrome b558. J. Immunol. 170: 6082 - 60892. Taylor, R. M. et al. (2004) Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit. J. Immunol. 173: 7349 - 7357 3. Taylor, R. M. et al. (2007) Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b. Mol. Immunol. 44: 625 - 637 4. Cao, S. (2010) Fc gamma receptors are required for NF-kappaB signaling, microglial activation and dopaminergic neurodegeneration in an AAV-synuclein mouse model of Parkinson's disease. Mol Neurodegener. 5:42.

Additional Info

Recognizes the native as well as the denatured form of the heavy chain of human Cytochrome B245. Epitope: aa498-506 (EKDVITGLK). Has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. Does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.

Alternative Names

Cytochrome b-245, beta Polypeptide, EC=1.-.-.-