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Cytochrome B5, Recombinant, Human

Cat no: C9095-30

Cytochrome B5, Recombinant, Human

Cytochrome b5 is a membrane-bound hemoprotein that enhances the catalytic efficiency of some P450 isoforms.\n\nSpecific Activity:\n52nmol heme per mg protein as determined by difference spectroscopy for reduced vs. oxidized protein. A millimolar extinction coefficient of 100mM-1 is used for the absorbance difference at 424 minus 490nm.\n\nQuality Control:\nMolecular weight and purity determined by SDS-PAGE. Protein is determined by the BCA assay with BSA as standard.\n\nStorage and Stability:\nStore at -70 degrees C for long term storage. Avoid repeated freeze/thaw cycles. Mix gently after thawing.

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SPECIFICATIONS

Catalog Number

C9095-30

Size

100ug

Form

Supplied as a liquid in 20mM Na/K phosphate, pH 7.25, 1mM EDTA.

Purity

(same/more than)90% by visual inspection of 5ug in a Coomassie stained SDS gel.

References

1. Holmans, et al., Arch. Biochem. Biophys. 312:554-565, 1994.

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