Cytochrome b5 Reductase 3, CYB5R3 (Diaphorase 1 (NADH), diaphorase 1, NADH-cytochrome b5 reductase, WU:Cyb5r3, WU:AL591952.1-003, WU:AL591952.1-002, WU:AL591952.1-001, Dia1, Dia-1, C85115, 2500002N19Rik, 0610016L08Rik, cytochrome b5 reductase 3, Cyb5r3)

Applications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 0.1-1ug/ml detects a ~35kD band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kD according to mouse NP_084063.1).\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9094-41

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1.J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70.

Additional Info

Recognizes mouse CYB5R3. Species sequence homology: rat, canine, bovine.

Alternative Names

diaphorase 1 (NADH), diaphorase 1, NADH-cytochrome b5 reductase, WU:Cyb5r3, WU:AL591952.1-003, WU:AL591952.1-002, WU:AL591952.1-001, Dia1, Dia-1, C85115, 2500002N19Rik, 0610016L08Rik, cytochrome b5 reductase 3, Cyb5r3