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Cytochrome C, Apo

Cytochrome C, Apo

Cat no: C9095-09A

Supplier: United States Biological
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Cytochrome C/Apaf-2 (apoptosis protease activating factor-2) is well known for its role in electron transport. During apoptosis (programmed cell death), Cytochrome C is released from the mitochondria into the cytosol where it binds to Apaf-1. Apaf-1 then activates caspase 9. Caspase 9 activation triggers other downstream caspase events in the proteolytic cascade. The mechanism by which Cytochrome C is released from the mitochondria and its function in Caspase 9 activation is still unclear. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1ug/ml Cell extracts from 293T cells (2x10e6) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with C9095-09A at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C9095-09A
Reactivities: Human, Mouse, Rat, Goat, Non-Human Primate, Sheep
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: ~95% (SDS-PAGE)
References: 1. Corradin, G., et al., J. Immunol. 133: 2915 (1984). 2. Li, P., et al., Cell 91: 479 (1997). 3. Reed, J.C., Cell 91: 559 (1997). 4. Yang, J., et al., Science 275: 1129 (1997). 5. Zou, H., et al., Cell 90: 405 (1997).
Additional info: Recognizes equine apo-Cytochrome C. Species Crossreactivity: human, mouse, rat, goat, sheep and monkey.

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