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Cytochrome C

Cytochrome C

Cat no: C9095-03

Supplier: United States Biological
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Cytochrome c is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis. Applications: Suitable for use in ELISA, Flow Cytometry, and Immunoprecipitation (native verified). Not suitable for Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation : 2ug/mg protein lysate. Native verified. Use Protein A. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C9095-03
Reactivities: Human, Bovine, Canine
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunoprecipitation
Size: 250ul
Form: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See C9095-03X
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein A affinity chromatography.
References: 1. Goshorn, S.C., et al. (1991) J. Biol. Chem. 266: 2134
Additional info: Recognizes equine cytochrome c (Mr 15kD). Species Crossreactivity: human, bovine, and canine.

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