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Cytochrome C (CYCS, CYC)

Cytochrome C (CYCS, CYC)

Cat no: C9095-02K

Supplier: United States Biological
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Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)0.5ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C9095-02K
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Additional info: Recognizes rat Cytochrome c. Species Crossreactivity: mouse and human.

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