Cytochrome C (CYCS, CYC)

Cat no: C9095-04J

Supplier: United States Biological

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Cytochrome C, approximately 13kD, highly conserved, is a heme- containing protein involved in mitochondrial electron transport. Cytochrome C is released from the mitochondria in response to pro- apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. Thus, Cytochrome C plays important roles in the activation of the DEVD-specific caspases such as Caspase-3 (CPP32) and DNA fragmentation. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:100 for 10min at RT Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C9095-04J
Reactivities:	Human
Hosts:	Rabbit
Applications:	Immunohistochemistry
Size:	50ug
Form:	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Hsu, Y. et al. Proc Natl Acad Sci USA 94, 3668 (1997). 2. Liu, X. et al. Cell 86, 147 (1997). 3. Hatefi, Y. et al. Ann Rev Biochem 54, 1015 (1985).
Additional info:	Recognizes human Cytochrome C.

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Cytochrome C (CYCS, CYC)

Cat no: C9095-04J

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