Cytochrome Oxidase Activity BioAssay(TM) Kit (Colorimetric)

Cytochrome c Oxidase (EC 1.9.3.1) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP.\n\nSample Type:\nPurified mitochondria and cells/tissue extracts. \n\nIntended Use:\nThe kit is useful for mitochondrial respiration studies, assembly of the complexes, mitochondria outer membrane integrity.\n\nTest Principle:\nCytochrome Oxidase Activity BioAssay(TM) Kit is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550nm.\n\nKit Components:\nCytochrome Oxidase Assay Buffer, 1x25ml\nEnzyme Dilution Buffer, 1x8ml\n1M DTT, 1x1ml\nCytochrome c, 2x vials\n96-well Plate, 1x plate\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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