Cytochrome P450 19A1 (Cytochrome P450 Family 19 Subfamily A Polypeptide 1, CYP19A1, CYP19, CYPXIX, Aromatase, ARO, ARO1, ARO 1, CPV1, CYAR, Estrogen Synthetase, MGC104309, P450AROM, P-450AROM)

P450 arom plays an important role in oestrogen biosynthesis and is highly conserved amongst mammals. P450 arom is highly expressed in placental tissue. The cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid Arachadonic Acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as mNADPH and P-450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). \n\nApplications:\nSuitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:250. Detects a band at ~55kD. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western Blots using this antibody.\nImmunohistochemistry: Paraffin sections. Does not require antigen retrieval using heat reatment prior to staining of paraffin sections.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice. \n\nRecommended Positive Control:\nHuman placenta\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9095-19W2

Size

1ml

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Goat, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Turner, K. J. et al (2002) J. Endocrinol. 172(1): 21-30. 2. Lu, Y. et al. (2007) Mol Endocrinol. 21: 651-63. 3. Zhao, D. et al. (2010) Nature. 464: 237-42. 4. Sirianni, R. et al. (2009) J Biol Chem. 284: 28905-16. 5. Carpino, A. et al. (2007) Eur. J. Endocrinol. 157: 239-244. 6. Catalano, S. et al. (2010) J Biol Chem. 285: 5581-93. 7. Wu, Y.G. et al. (2011) Mol Endocrinol. 25: 656-68. 8. Lu, Y. et al. (2011) J Steroid Biochem Mol Biol. 123: 71-8. 9. Barone, I. et al. (2009) Cancer Res. 69: 4724-32. 10. Ghosh, S. et al. (2008) Int J Biomed Sci. 4: 260-265.11. Jeong, J.H. et al. (2010) Mol Cell Biol. 30: 2365-75.12. Rago, V. et al. (2005) Reprod Biol Endocrinol. 3: 72.13. Schmidt, M. et al. (2005) Arthritis Res Ther. 7: R938-48.

Additional Info

Recognizes a conserved epitope within human Cytochrome P450 aromatase (P450 arom). Species Crossreactivity: rat, chicken, giraffe, mouse, porcine, baboon, bovine, equine, rabbit, sheep, goat, great fruit eating bat, collared peccary and marmoset.