Cytochrome P450 26A1 (CYP26A1)

Cytochrome P450 enzymes, a superfamilyof b-type heme-containing proteins found in organisms from alldomains of life, are major catalysts in the oxidative transformationof a diversity of endogenous and exogenous compounds. CYP enzymes play an important role in the metabolic activation of environmental procarcinogens or chemical carcinogenesis, these enzymes are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. CYP26A1 is a 497aa enzyme in mouse, rat and human (chr 10q23) and a distinct composite retinoic acid response element underlies the complex regulation of retinoic acid metabolism. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in the regulation of gene expression in both embryonic and adult tissues. Highest levels of expression are noticed in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100,000; using 50-100ng control peptide/well. See C9095-29E.\nWestern Blot: 1-10ug/ml using Chemiluminescence\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9095-29D

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Niederreither, K et al (2002) Nature Genet. 31: 84-88; 2. White J. A et al (1997) JBC. 272: 18538-18541; 3. White , J. A et al ( 1998) Genomics 48: 270-272; 4. McSorley, L. C et al (2000) Biochem Pharmacol. 60 (4), 517-526.

Additional Info

Recognizes human Cytochrome P450 26A1 (CYP26A1).