The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA?s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET?s, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET?s are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET?s are converted from AA by the 2C11 family of P450?s whose expression is induced by testosterone and is therefore not generally found in females.
Applications:
Suitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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