Cytochrome P450 2D6, Recombinant, Human (CYP2D6)

Cytochrome P450 2D6 is a member of a complex family of microsomal enzymes (mono-oxygenases) present in the endoplasmic reticulum membrane, which perform detoxification reaction on xenobiotic compounds. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1) which has been classically defined by immunofluorescence microscopy. The presence of these autoantibodies is considered indicative of Autoimmune Hepatitis Type 2; LKM 1 antibodies have also been detected in patients with hepatitis C viral infection. The International Autoimmune Hepatitis Group therefore has subdivided the AIH type 2 into two subgroups: 2a with HCV and 2b without HCV. AIH 2a patients are often over 40 and predominantly male. The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques. Recombinant Human Cytochrome P450 2D6, also called liver/kidney microsomal antigen 1is produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 55,801 dalton. Recombinant LKM 1 is purified by proprietary chromatographic techniques.\n\nImmunological Functions:\n1. Binds IgG-type human auto-antibodies.\n2. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).\n\nApplications: \nWestern-Blot with monoclonal anti-LKM-1 antibody and autoimmune hepatitis patient sera.\n\nCoating Concentration:\n0.35-0.6ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\n\nStorage Conditions: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees Cfor longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C9095-15K2

Size

5ug

Form

Supplied as a liquid in 16mM sodium phosphate buffer, pH 7.6, 400mM sodium chloride, 0.08mM KCl and 20% glycerol.

Purity

(same/more than) 40% (SDS-PAGE)

References

1. Mechanism-based inhibition of human liver microsomal cytochrome P450 2D6 (CYP2D6) by alkamides of Piper nigrum.\n\nPlanta Med 2006 May;72(6):527-32\n\n2. Drug disposition of chiral and achiral drug substrates metabolized by cytochrome P450 2D6 isozyme: case studies, analytical perspectives and developmental implications.\n\nBiomed Chromatogr 2006 Jun;20(6-7):466-91\n\n3. Acute dystonic reaction to metoclopramide in patients carrying homozygous cytochrome P450 2D6 genetic polymorphisms.\n\nNeth J Med 2006 May;64(5):160-2\n\n4. In Silico Prediction of Drug Binding to Cytochrome P450 2D6: Identification of a new Metabolite of Metoclopramide.\n\nDrug Metab Dispos 2006 May 12;\n\n5. New cytochrome P450-2D6*56 allele identified by genotype/phenotype analysis of cryopreserved human hepatocytes.\n\nDrug Metab Dispos 2006 May 5;\n\n6. The association between cytochrome P450-2D6 genotype and prescription of antiparkinsonian drugs in hospitalized psychiatric patients using antipsychotics: a retrospective follow-up study.\n\nJ Clin Psychopharmacol 2006 Apr;26(2):212-5