Cytochrome P450 2E1

Cytochrome P450 is mainly found in liver, and can metabolize a wide range of compounds such as organic solvents, acetaminophen, dimethylnitrosoamine and aliphatic alcohol, which have certain toxicological effects in humans. Ethanol-induced cytochrome P450 IIE1 is considered to be one of the major causes of oxidative stress in the liver following ethanol treatment.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 will yield a positive result with (same/more than)0.2ug of recombinant human cytochrome P450 2E1 using alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody.\nOptimum dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9095-17W

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 20mM phosphate buffer, pH 8.0.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Gillarm, E.M., Guo, Z. and Guengerich, F.P., Arch. Biochem. Biophys. 312: 59-66 (1994).

Additional Info

Recognizes a band (P450 2E1) in human liver microsomes. Reactive with (same/more than)0.2ug of recombinant human P450 1A2. Only slightly crossreacts with recombinant human P450 1A1 after prolonged incubation. Does not crossreact with recombinant human cytochromes P450 2E1, 3A4 or 2C10.