Cytochrome P450 46A1, CT (CYP46A1, Cholesterol 24-hydroxylase, CH24H, CYP46)

CYP46A1 converts cholesterol into the biologically active oxysterol and 24(S)-hydroxycholesterol, in the first step of the enzymatic degradation of cholesterol in the brain. CYP46A1 is also thought to play a role in the metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system.\n\nP450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members: CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:15,000\nWestern Blot: 1:3,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9095-46

Size

100ul

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Pikuleva IA. (2006) Cytochrome P450s and cholesterol homeostasis. Pharmacol Ther. 112:761-73. 2. Mast, N. et al. (2003) Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain. Biochemistry. 42:14284-14292. 3. Ng, P. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s. Drug Metabol. Pharmacokin. 18(3):163-172.

Additional Info

Recognizes human cholesterol 24-hydroxylase (CYP46A1).