Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. Cytokeratin 10 and 13 are members of the type I (acidic) cytokeratin family. Cytokeratin 10 is synthesized in the suprabasal cell layers of certain stratified epithelia, in an endogenous differentiation program and is expressed in certain epithelial tumors and is generally associated with keratin-1. Its deficiency leads to epidermolytic hyperkeratosis (EHK). Cytokeratin 13 is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in Cytokeratin 13 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Gene Bank Accession No: NP_000412.2 (KRT10), Gene Bank Accession No: NP_705694.1 (KRT13)
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:100-1:200
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Skin
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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