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Cytokeratin 10

Cat no: C9097-11A

Cytokeratin 10

Cytokeratin 10 (CK10) is a heterotetramer of two type I and two type II keratins. CK10 is typically associated with cytokeratin 1 (CK1). CK10 is expressed in all suprabasal cell layers and it is part of acidic/high molecular weight keratin subfamily. Defects in CK10 causes Epidermolytic hyperkeratosis (EHK), a hereditary skin hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. CK10 has also been linked to cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. CK10, along with CK14, 15, 16, and 20, can be used to determine if a neoplasm originated from an epithelial or non-epithelia origin, such as the determination of metastatic malignant tumors of epithelial origin.\n\nApplications:\nSuitable for use in Indirect Immunoperoxidase staining, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Pretreatment of formalin fixed sections with protease type XXIV (500ug/ml) produces best results.\nIndirect Immunoperoxidase: Identifies cytokeratin expressing cells on frozen sections.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-11A

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 1% BSA, 10mM sodium azide.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation followed by ion exchange chromatography.

Isotype

IgG1

References

1. Markley A et al., J Invest Dermatol 97:763-70 (1991) 2. Brockaert D, et al. Arch Dematol Res 1990; 282 :383

Additional Info

Recognizes human cytokeratin 10 found in suprabasal cells of stratified squamous epithelia.

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