Cytokeratin 10 (CK10) is a heterotetramer of two type I and two type II keratins. CK10 is typically associated with cytokeratin 1 (CK1). CK10 is expressed in all suprabasal cell layers and it is part of acidic/high molecular weight keratin subfamily. Defects in CK10 causes Epidermolytic hyperkeratosis (EHK), a hereditary skin hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum (1). CK10 has also been linked to cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. CK10, along with CK14, 15, 16, and 20, can be used to determine if a neoplasm originated from an epithelial or non-epithelia origin, such as the determination of metastatic malignant tumors of epithelial origin (2).
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:50,000-100,000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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