Cytokeratin 14

Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells. \n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\n\\Immunohistochemistry: Suitable for staining formalin fixed wax sections or frozen tissues. Recommended to either microwave treat fixed tissue sections or high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formal saline \nOptimal dilutions to be determined by researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice.\n\nSource: Tissue culture supernatant \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-14B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as liquid in PBS, 10mM sodium azide, 1mg/ml BSA.

P Type

Mab

Purity

Purity by ammonium sulfate precipitation followed by ion exchange chromatography.

Isotype

IgG3

References

US Biological application references: 1. Zhang, C. et al., (2011) PLoS ONE 6(8): e23849. doi:10.1371/journal.pone.0023849 2. Dakir, E. H. et al., (2008) Carcinogenesis 28:2377-2384. 1. Moll, R., et al., Cell 31: 11-14 (1982). 2. Purkis, P.E., et al., J. Cell Sci. 97: 39-50 (1990).

Additional Info

Recognizes human Cytokeratin 14.