Cytokeratin 14 (KRT14, Keratin 14, CK14, EBS3, EBS4, K14, Keratin, type I cytoskeletal 14, NFJ) (FITC)

Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells. \n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul per test.\nImmunohistochemistry (Formalin fixed paraffin sections or frozen tissues): Recommended to either microwave treat fixed tissue sections or use high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formalin.\nOptimal dilutions to be determined by researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice.\n\nSource:\nTissue culture supernatant \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-14D

Size

100Tests

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as liquid in PBS, 0.1% sodium azide, 0.1% BSA. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purity by ammonium sulfate precipitation followed by ion exchange chromatography.

Isotype

IgG3

References

1. Moll, R., et al., Cell 31: 11-14 (1982). 2. Purkis, P.E., et al., J. Cell Sci. 97: 39-50 (1990). 3. Wang, D., et al., Invest. Opthamol. Vis. Sci. 44: 4698-4704 (2003).

Additional Info

Recognizes human Cytokeratin 14.