Cytokeratin 14, Recombinant, Human

Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.\n\nRecombinant Human Cytokeratin 14 is a single,non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nNote: 0.1% BSA or HSA is recommended for long term storage.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-14R

Size

5ug

Form

Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM EDTA, 10mM methylammonium chloride. Reconstitute with sterile ddH2O to a concentration >100ug/ml, which can then be further diluted to other aqueous solutions. 0.1% BSA or HSA is recommended for long term storage.

Purity

(same/more than) 95% by RP-HPLC or SDS-PAGE. Chromatographically purified.

References

1. Reis-Filho, J.S., et al., Virchows Arch. 443(2): 122-132 (2003). 2. Vaidyanathan, S., et al., Spinal Cord 41(8): 432-434 (2003). 3. de Araujo, V.C., et al., Histopathology 41(3): 244-249 (2002). 4. Southern, S.A. & McDicken, I.W., Hum. Pathol. 32(12): 1351-1355 (2001). 5. Chu, P.G. & Weiss, L.M., Histopathology 39(5): 455-462 (2001). 6. Chu, P.G. & Lyda, M.H., Histopathology 39(1): 9-16 (2001).