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Cytokeratin 15 (Keratin 15, KRT15, CK15, CK-15, Cytokeratin-15, K15, K1CO, Keratin, type I cytoskeletal 15, KRTB)

Cat no: C9097-15B

Cytokeratin 15 (Keratin 15, KRT15, CK15, CK-15, Cytokeratin-15, K15, K1CO, Keratin, type I cytoskeletal 15, KRTB)

Keratins is a family of about 20 proteins that form intermediate filament in epithelial cells. Obligate heteropolymers of a type I and a type II subunit, they contribute to the structure and strength of the cytoskeleton. Mutations in keratin genes cause keratinopathies, inherited diseases of the skin and its appendages. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant intermediate filament protein complement in an epithelial cell-specific manner. Cytokeratin 15 belongs to the acidic or type I keratin family and assembles into a keratin filament network paired with K5, its basic, type II keratin expression partner. It is expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail Human K15 is encoded on chromosome 17q21.2.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-15B

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. Wascern et al. J Invest Dermatol 112: 362, 1999. 2. Radoja et al. Mol Cell Biol 24: 3168, 2004.

Additional Info

Recognizes human Cytokeratin 15. Species Crossreactivity: bovine, mouse and rat.

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