Cytokeratin 16

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nFusion Partners: Spleen cells from immunized mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.\n\nRecommended Positive Control Tissue: Non-melanocytic skin cancer, basal cell carcinoma\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nImmunohistochemistry: (frozen)- 1:20-1:40, (paraffin)- 1:20 -1:40\nOptimal working dilutions to be determined by researcher.\n\nImmunohistochemistry: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH 6.0 is recommended for this purpose.

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SPECIFICATIONS

Catalog Number

C9097-15N

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Tissue Culture Supernatant lyophilized with 0.1% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG1

References

1. Markey, A.C. et al. (1992). Keratin expression in basal cell carcinomas. Br. J. Dermatol. 126: 154-160.2. Wetzels, R.H.W. et al. (1991). Basal cell-specific and hyperproliferation related keratins in human breast cancer.

Additional Info

Recognizes human cytokeratin 16, expressed in areas where there is high turnover of keratinocytes. Cytokeratin 16 expression is particularly pronounced in conditions such as wound healing, psoriasis and also in some carcinomas such as basal cell carcinoma.