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Cytokeratin 16 (Keratin, Type I Cytoskeletal 16, Cytokeratin-16, CK-16, Keratin-16, K16, KRT16, KRT16A)

Cat no: C9097-15P3

Cytokeratin 16 (Keratin, Type I Cytoskeletal 16, Cytokeratin-16, CK-16, Keratin-16, K16, KRT16, KRT16A)

Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. Keratin 16 is a type-I keratin which associates with KRT6 isomers. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. It maps to 17q12-q21 region of human chromosome. It is expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis and also found in luminal cells of sweat and mammary gland ducts. Defects in KRT16 are a cause of pachyonychia congenita type 1, nonepidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50\nImmunohistochemistry (frozen): 1:20-1:40\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-15P3

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Markey et al. Br J Dermatol 126: 154, 1992. 2. Wetzels et al. Am J Pathol 138: 751, 1991.

Additional Info

Recognizes human Cytokeratin 16.

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