Cytokeratin 17

Applications:\nSuitable for use in Immunohistochemistry (frozen tissue and paraffin-embedded tissue when using the microwave method) and Immunoblot (Western).\n\nRecommended Dilution: \nImmunohistochemistry: 1:10; Incubation Time 1hr at RT.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-17B

Size

50ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Reconstitute to a final volume of 1ml.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

Guelstein V.I., Tchypysheva T.A., Ermilova V.D., Litvinova L.V., Troyanowsky S.M. and Bannikov G.A.: Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer. Int. J. Cancer 42, 147-153 (1988)\nTroyanowsky S.M., Guelstein V.I., Tchypysheva T.A., Krutovskikh V.A. and Bannikov G.A.: Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J. Cell Sc. 93, 419-426 (1989)\nSmedts F., Ramaekers F., Troyanowsky S., Pruszcynski M., Robben H., Lane B., Leigh I., Plantema F. and Vooijs P.: Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia. Am. J. Pathol. 140, 601-612 (1992)\nMoll, I. and Moll, R.: Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300-309 (1991)\nDe Jong E. van Vlijmen I., van Erp P. Ramaekers F., Troyanowsky S. and Van de Kerkhof P.: Monoclonal anti-keratin 17: A useful marker for anti-psoriatic therapies. Arch. Dermatol. Res. 283, 480-482 (1991)\nWetzels R.H.W., Schaafsma H.E., Leigh I.M., Lane E.B., Troyanowsky S.M., Wagenaar S.S., Vooijs, G.P., Ramaekers F.C. S.: Type VII collagen in human lung carcinomas. Correlation with expression of keratins 14, 16, 17 and 18. Histopathology (in press)\nDemirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).\nMoll R. et al.: Differenzierungsmarker bei gyn

Additional Info

Antigen Recognized in Human, rat (Mr 40,000 polypeptide). Reactivities on Cultured HeLa, A-431. Tumors Specifically Detected Benign tumors of mammary gland; most malignant forms of mammary tumors are negative for cytokeratin 17. Represents an excellent marker to distinguish myoepithelial cells from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors.