Cytokeratin 17, NT (Cytokeratin-17, CK 17, CK-17, K17, Keratin 17, Keratin-17, Keratin Type I Cytoskeletal 17, KRT 17, KRT17, KRT17 Protein, PC, PC2, PCHC1)

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). KRT17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10 \nWestern Blot: 1:2000 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:50-1:100 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-16H

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Troyanovsky SM, et al. Eur J Cell Biol 59(1):127-37, 1992. 2. Kim S, et al. Nature 441(7091):362-5, 2006. 3. Covello SP, et al. Br J Dermatol 139(3):475-80, 1998.

Additional Info

Recognizes human Cytokeratin 17. Species Crossreactivity: Rat.