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Cytokeratin 18

Cat no: C9097-20G

Cytokeratin 18

Represents an excellent marker for all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1:2000 (ECL).\nImmunohistochemistry (Frozen): 1:200; Incubate 1 hr at RT.\nImmunohistochemistry (Paraffin-embedded tissue): 1:100 \nIncubate > 1 hr at RT; extended time for paraffin sections.\nOptimal dilution determined by the researcher. \n\nPositive Controls:\nTumors Specifically Detected: All adeno-Ca tested, undifferentiated CA, hepatocellular CA, cervix Ca.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-20G

Size

100ul

Applications

ICC, IHC, WB

Reactivities

Hum, Mouse, Bov

Form

Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Magin, T.M., et al., Eur. J. Cell Biol. 53: 333-348 (1990).

Additional Info

Recognizes acidic human cytokeratin 18 (Mr 45,500). No crossreaction with other keratins and intermediate filament proteins. Species Crossreactivity: mouse and bovine.

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