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Cytokeratin 19

Cat no: C9097-27B


Supplier: United States Biological
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Applications: Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:10 Suitable for use on frozen tissue, on paraffin-embedded tissues (microwave procedure highly recommended) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C9097-27B
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a Lyophilized powder from PBS, pH 7.4 containing 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml ddH20, 40-50% glycerol.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A chromatography
Additional info: Recognizes Cytokeratin 19. No reaction with hepatocellular carcinoma. Reactive with cytokeratin 19 rod fragment. Polypeptide reacting: Mr 40,000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on a.a. 311-335 (QSQLSMKAALEDTLAET EARFGAQL) of the alpha-helical fragment. No reaction with hepatocellular carcinoma. Tumors specifically detected: All tested adenocarcinoma; cholangio ca. of liver; renal cell carcinoma; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma.

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