Cytokeratin 19 (Keratin, Type I Cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19)

Supplier:

United States Biological

Cat no: C9097-24U

Cytokeratin 19 (Keratin, Type I Cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19)

Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. Cytokeratin 19 (K19) is a type I intermediate filament protein that is expressed in stratified and simple-type epithelia. It is unique in that it has a very short 13aa tail domain. It possesses assembly properties and it may impart unique properties to the basal cells expressing it in skin epithelia. It maps to the 17q21.2 region oh human chromosome and has a molecular weight of 44,098D. It is expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:25-1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-24U

Size

500ul

Applications

ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Bartek et al. J Cell Sci 75: 17, 1985. 2. Bartek et al. Histochem J 18: 565, 1986. 3. Bartek et al. Int J Cancer 36: 299, 1985. 4. Bartek et al. J Cancer Clin Oncol 22: 1441, 1986 5. Yuan et al. Apoptosis 3: 161, 1998. 6. Zhou et al. J Biol Chem 274: 12861, 1999. 7. Eckert et al. Proc. Natl. Acad. Sci. 85: 1114, 1988.

Additional Info

Recognizes human Cytokeratin 19.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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