Cytokeratin 20

Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. \n\nApplications: \nSuitable for use in Immunohistochemistry (paraffin sections after microwave treatment) and Immunoblot (Western).\n\nRecommended Dilution: \nImmunohistochemistry: 1:10 in PBS (pH 7.4) (1:100 when applying the microwave method); Incubation Time 1 hr at RT or overnight at 2-8 degrees C.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-29D

Size

50ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute to a final volume of 1ml.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Moll, R., L

Additional Info

Antigen Recognized in Human; not with rat and mouse. Reactivities on Cultured HT-29, LoVo, DLD-1, SW 1116. Polypeptide recognized: protein IT (cytokeratin No. 20; Mr 46,000).\nCell Lines (tested so far) CaCo-2, RT-4.