Cytokeratin 5

CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunohistochemistry: 1:200-1:1000.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

030135

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Ascites fluid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Canedo-Patzi AM. Leon-Bojorge B. de Ortiz-Hidalgo C. Gac Med Mex. 2006, Jan-Feb, 142(1):59-66. Spanish.\n2. Leibl S. Moinfar F. Am J Surg Pathol. 2006, Apr, 30(4):450-6.\n

Additional Info

Human

Alternative Names

K5; DDD; EBS2; KRT5A; KRT5