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Cytokeratin 5 + 14

Cytokeratin 5 + 14

Cat no: C9097-37R

Supplier: United States Biological
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Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70kD and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. Cellular Localization: Cytoplasmic Positive Control: Skin Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr) and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C9097-37R
Reactivities: Human
Hosts: Mouse
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 6ml
Form: Supplied as a liquid in PBS, pH 7.2.
P type: Mab
Isotype: IgG1+IgG3
Purity: Purified
References: 1. Irvine et al. (1997) J Invest Dermatol 108:809. 2. Whittock et al. (2000) Biochem Biophys Res Commun 274:149. 3. Lane et al. (1990) Can Biol 1:165. 4. Moll et al. (1982) Cell 31:11. 5. Purkis et al. (1990) J Cell Sci 97:39. 6. Westzels et al. (1989) Am J Pathol 134:571.
Additional info: This antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50kD cytokeratin protein designated cytokeratin 14. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58kD protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified epithelial cells from simple epithelial cells. Species Crossreactivity: This antibody is reactive against human.

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