Cytokeratin 5, 8 (Keratin 5, 8, Keratin Type II Cytoskeletal 5, Keratin 5, Keratin-5, K5, KRT5, KRT5A, Keratin Type II Cytoskeletal 8, Keratin 8, Keratin-8, K8, KRT8, 58kD Cytokeratin, CARD2, Cytokeratin-5, CK5, CK-5, Cytokeratin-8, CK8, CK-8, CYK8, DDD,

Keratin is a family of about 20 proteins that form intermediate filament in epithelial cells. Obligate heteropolymers of a type I and a type II subunit, they contribute to the structure and strength of the cytoskeleton. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Cytokeratin 5 is a type-II keratin specifically expressed in the basal layer of the epidermis with family member cytokeratin 14. The pathological role of cytokeratin 5 has been identified in epidermolysis bullosa simplex, epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) and Dowling-Degos disease. It maps to 12q12-q13 region of human chromosome. Keratin 8, Cytokeratin 8 is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Together with cytokeratin 19, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Mutations in this gene have been linked to liver cirrhosis. It maps to 12q13 region of human chromosome.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100-1:300\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:100\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-37H5

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Bartek et al. J Pathol 164: 215, 1991. 2. Angus et al. J Pathol 153: 377, 1987. 3. Krauss et al. Gene 86: 241, 1990. 4. Ku N.-O.et al. Proc Natl Acad Sci U.S.A 100: 6063, 2003.

Additional Info

Recognizes human cytokeratins 5 (58kD) and 8 (52.5kD).