Cytokeratin 7

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. This antibody reacts particularly well in paraffin embedded tissue. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: Frozen tissue after fixation in methanol. Paraffin embedded tissue sections pretreated with 0.1% pronase/ 0.1% trysin in PBS or 0.5% pepsine in 0.01N HCl. Avidin-biotinylated horseradish peroxidase complex as a detection reagent. 1:50-1:100 (ABC).\nWestern Blot: 1:50-1:500\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nHybridization of SP2/0-Ag14 myeloma cells with spleen cells from mice immunized with cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-39A

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Niekerk CC van, Jap PHK, Ramaekers FCS, Molengraft F van de, Poels LG. Immunohistochemical demonstration of Keratin 7 in routinely fixed paraffin-embedded human tissues. J. Pathol. 165: 145-152 (1991). 2. Niekerk CC van, Boerman OC, Ramaekers FCS, Poels LG - Marker profile of different phases in the transition of normal human ovarian epithelium to ovarium carcinomas. Am. J. Pathol. 138: 455-463 (1991). 3. Molengraft F. van de, Niekerk CC van, Jap PHK, Poels LG - OV-TL12/30 (keratin antibody) is a marker of glandular differentiation in lung cancer. Histopathology 22: 35-38 (1993). 4 Niekerk CC van, Vooijs P, Casparie-van Velsen IJ, Poels LG - Differentiatio margins of ovarian tumor pathology: First incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detection and Prevention 21: 247-25 (1997). 5. Torenbeek R, Lagendijk JH, Diest PJ van, Bril H, Molengraft FJJM van de, Meijer CJLM - Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma. Histopathology 32: 20-27 (1998).

Additional Info

Recognizes human Cytokeratin 7 (exclusively). Reacts with a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. \n