Cytokeratin 7 (Cytokeratin-7, CK 7, CK7, CK-7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin-7, K7, KRT7, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type-II Keratin Kb7, Type II

Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. \n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul per test\nImmunocytochemistry: 1:10 using isolated EVT cells fixed with 0.5% PFA at 4 degrees C for 15 minutes followed by acetone at -20 degrees C for 2 minutes.\nImmunohistochemistry (Paraffin embedded sections): Protease treatment of paraffin sections is required.\nImmunohistochemistry (Frozen): 1:10 used with placental tissue (7um) fixed with acetone for 1 hour.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nSource: Tissue culture supernatant\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C9097-40D1

Size

100Tests

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by ammonium sulfate precipitation followed by ion exchange chromatography.

Isotype

IgG2b

References

1. Osborn, M. et al. Lab. Invest. 55, 497-504 (1986). 2. Moll, R. et al Cell 31, 11-24 (1982). 3. Ramaekers, F. et al. Path. 11, 14-17 (1990). 4. Vojtesek, B. et al. Neoplasma 37, 333-42 (1989).

Additional Info

Recognizes human Cytokeratin 7.