Cytokeratins are a family of water-insoluble intermediate ?laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci?c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi?cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.
Applications:
Suitable for use in Immunohistochemistry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions:
Western Blot: Detects a band at ~52.2kD (originally reported also as 45 kD cytokeratin 18 in the Moll-catalogue).
Immunohistochemistry: 1:5-1:10 frozen and paraffin-embedded tissue require Proteolytic treatment with pepsin or pronase.
Immunocytochemistry: Cell Supensions require Proteolytic treatment with pepsin or pronase.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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