Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD, Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO)

Applications: \nSuitable for use in Immunocytochemistry, Immunohistochemistry (frozen sections) and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nImmunocytochemistry: 1:5-1:10\nImmunohistochemistry: 1:5-1:10\nNote: Dilute antibody immediately before use. Incubation Time 1 hr at room temperature.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-42F1

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid containing 0.1% sodium azide

P Type

Mab

Purity

Culture supernatant

Isotype

IgG1

References

1. Edwards BF et al : Transitional cell cytokeratins as a second parameter in flow cytometry of bladder cancer (1987). World Journal Urology 5:123-127. 2. Muijen GNP van et al : Coexpression of intermediate filament polypeptides in human fetal and adult tissues (1987). Lab Invest 57,,359-369. 3. Muijen GNP van et al : Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in foetal, newborn and adult epidermis (1987). Exp Cell Research 171:331-345. 4. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)

Additional Info

Antigen Recognized Human, mouse, rat, rabbit (most mammals) in Species (tested so far). Recommended for Glandular epithelia. In immunoblot, the antibody is directed against the 52.2kD cytokeratin protein (cytokeratin 8). Exclusively reactive with cytokeratin 8. Cytokeratin 8 is present in simple type epithelia, in glandular epithelium and in most tumors derived therefrom. Epitope has been mapped to aa353-367 on alpha helical rod domain.