Cytokeratin 9

CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oe-sophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. (see references listed below).\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded cells and tissue).\n\nRecommended Dilution: \nImmunohistochemistry: 1:100-1:200; 1:50 for paraffin-embedded tissue; Incubation Time 1 hr at RT; extended with paraffin.\nWestern Blot: 1:5000-1:10,000 (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-43

Size

100ul

Applications

IHC, WB

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid, 0.5% BSA and 0.09% sodum azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Langbein L et al. Molecular characterization of the body site-specific human cytokeratin 9: cDNA cloning, amino acid sequence, and tissue specificity of gene expression. Differentiation 55:57-71 (1994)\n2. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)

Additional Info

Reactive Polypeptide: Acidic human cytokeratin 9 (MW 62,129), expressed in the palmoplantar epidermis. The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. \nSpecies Crossreactivity: Human, bovine, mouse and rat.